Journal article
Quantifying adaptive and innate immune responses in HIV-infected participants using a novel high throughput assay
MK Yong, PU Cameron, T Spelman, JH Elliott, CK Fairley, J Boyle, M Miyamasu, SR Lewin
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2016
Abstract
Objectives: HIV infection is characterised by persistent immune dysfunction of both the adaptive and innate immune responses. The aim of this study was to evaluate these responses using a novel high throughput assay in healthy controls and HIV-infected individuals prior to and following anti-retroviral treatment (ART). Design: Cross-sectional study. Methods: Whole blood was assessed using the QuantiFERON Monitor® (QFM) assay containing adaptive and innate immunostimulants. Interferon (IFN)-γ levels (IU/mL) were measured by enzyme-linked immunosorbent assay (ELISA). Results: We recruited HIV-infected participants (n = 20 off ART and viremic; n = 59 on suppressive ART) and HIV-uninfected contr..
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Awarded by National Health and Medical Research Council (NHMRC)
Awarded by National Institutes for Health
Funding Acknowledgements
This investigator initiated industry funded study was supported in part by QIAGEN. The study design, statistical analysis and preparation of the manuscript were done independent of the sponsor. MKY was awarded a PhD Scholarship by the National Health and Medical Research Council (NHMRC 0607212). SRL is an NHMRC Practitioner Fellow. SRL and PUC are supported by the National Institutes for Health U19 AI096109. JB and MM are employees of the sponsor but did not have any additional role in the study design, analysis, decision to publish or preparation of the original manuscript.